Question 1 · structured
10 marksA student investigates the effect of sucrose concentration on potato tissue. They cut five potato cylinders to equal lengths of 5.0 cm and place each one in a test-tube containing a different concentration of sucrose solution. After 2 hours, they measure the change in mass of each cylinder and calculate the percentage change.
The results are shown in the table:
| Concentration of sucrose solution / \(mol/dm^3\) | Percentage change in mass / \% |
| :---: | :---: |
| 0.0 | +12.5 |
| 0.2 | +6.0 |
| 0.4 | -1.5 |
| 0.6 | -8.0 |
| 0.8 | -14.0 |
(a) State and explain the percentage change in mass of the potato cylinder in the \(0.0\text{ }mol/dm^3\) sucrose solution. [3]
(b) State the name of the process that causes water to enter or leave the potato cells. [1]
(c) Use the data in the table to estimate the sucrose concentration inside the cells of the potato tissue. Explain how you arrived at your estimate. [2]
(d) Explain, in terms of turgor and cell wall pressure, what happens to the cells of the potato cylinder placed in the \(0.8\text{ }mol/dm^3\) sucrose solution. [3]
(e) State one variable, other than sucrose concentration, that must be controlled in this investigation. [1]
The results are shown in the table:
| Concentration of sucrose solution / \(mol/dm^3\) | Percentage change in mass / \% |
| :---: | :---: |
| 0.0 | +12.5 |
| 0.2 | +6.0 |
| 0.4 | -1.5 |
| 0.6 | -8.0 |
| 0.8 | -14.0 |
(a) State and explain the percentage change in mass of the potato cylinder in the \(0.0\text{ }mol/dm^3\) sucrose solution. [3]
(b) State the name of the process that causes water to enter or leave the potato cells. [1]
(c) Use the data in the table to estimate the sucrose concentration inside the cells of the potato tissue. Explain how you arrived at your estimate. [2]
(d) Explain, in terms of turgor and cell wall pressure, what happens to the cells of the potato cylinder placed in the \(0.8\text{ }mol/dm^3\) sucrose solution. [3]
(e) State one variable, other than sucrose concentration, that must be controlled in this investigation. [1]
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Worked solution
(a) Mass increased by \(12.5\%\); because the water potential outside the potato tissue was higher than inside the potato cells, causing water to move into the cells down a water potential gradient by osmosis.
(b) Osmosis.
(c) Between \(0.2\) and \(0.4\text{ }mol/dm^3\) (specifically around \(0.36\text{ }mol/dm^3\)); because this is the concentration where there is no percentage change in mass, meaning there is no net movement of water into or out of the cells (isotonic point).
(d) Water moves out of the potato cells by osmosis down a water potential gradient; the vacuole and cytoplasm shrink, causing turgor pressure to drop; the cell membrane pulls away from the cell wall, leaving the cells flaccid or plasmolysed.
(e) Temperature / potato variety / duration of immersion / surface area of potato cylinders.
(b) Osmosis.
(c) Between \(0.2\) and \(0.4\text{ }mol/dm^3\) (specifically around \(0.36\text{ }mol/dm^3\)); because this is the concentration where there is no percentage change in mass, meaning there is no net movement of water into or out of the cells (isotonic point).
(d) Water moves out of the potato cells by osmosis down a water potential gradient; the vacuole and cytoplasm shrink, causing turgor pressure to drop; the cell membrane pulls away from the cell wall, leaving the cells flaccid or plasmolysed.
(e) Temperature / potato variety / duration of immersion / surface area of potato cylinders.
Marking scheme
(a)
- Mass increased / gained mass [1]
- Water potential is higher outside the tissue than inside [1]
- Water moves in by osmosis [1]
(b)
- Osmosis [1]
(c)
- Estimate in the range of 0.35 to 0.37 \(mol/dm^3\) (or state between 0.2 and 0.4 \(mol/dm^3\)) [1]
- Explanation: No net movement of water / no change in mass occurs here [1]
(d)
- Water leaves cells by osmosis / vacuole shrinks [1]
- Loss of turgor pressure / cells become flaccid / flaccid state [1]
- Plasmolysis / cell membrane pulls away from cell wall [1]
(e)
- Temperature / variety of potato / duration of immersion [1]
- Mass increased / gained mass [1]
- Water potential is higher outside the tissue than inside [1]
- Water moves in by osmosis [1]
(b)
- Osmosis [1]
(c)
- Estimate in the range of 0.35 to 0.37 \(mol/dm^3\) (or state between 0.2 and 0.4 \(mol/dm^3\)) [1]
- Explanation: No net movement of water / no change in mass occurs here [1]
(d)
- Water leaves cells by osmosis / vacuole shrinks [1]
- Loss of turgor pressure / cells become flaccid / flaccid state [1]
- Plasmolysis / cell membrane pulls away from cell wall [1]
(e)
- Temperature / variety of potato / duration of immersion [1]