問 1
1 点In genetic fingerprinting, DNA fragments are separated using gel electrophoresis. Which principle explains the movement of these fragments through the gel?
AQA A Level · Biology 7402
Gene technologies allow the study and alteration of gene function allowing a better understanding of organism function and the design of new industrial and medical processes：練習問題
その場で採点される選択問題 5 問と、解説つきの記述問題 3 問。すべて「Gene technologies allow the study and alteration of gene function allowing a better understanding of organism function and the design of new industrial and medical processes」からの出題です。
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問 2
1 点In the Polymerase Chain Reaction (PCR), primers are essential components of the reaction mixture. What is their specific function?
問 3
1 点In recombinant DNA technology, what is the specific role of DNA ligase during the formation of a recombinant plasmid?
問 4
1 点A scientist is using DNA probes and hybridisation to screen a patient for a specific heritable disease. Which of the following is a necessary step in this process?
問 5
1 点During recombinant DNA technology, why can a fragment of DNA from a human gene be successfully translated into a protein within a transgenic bacterium?
問 6
3 点In recombinant DNA technology, the enzyme reverse transcriptase is frequently used to produce DNA fragments from mRNA.
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}Explain one advantage of using mRNA as the starting material for obtaining a gene, rather than using a sample of the organism's total nuclear DNA.
まず自分で答えを書いてから、解説と照らし合わせましょう。
問 7
5 点A researcher uses DNA probes and DNA hybridisation to locate a specific mutant allele associated with a genetic disorder.
Explain why it is necessary to denature the patient's double-stranded DNA before the labelled probe can be used effectively.
まず自分で答えを書いてから、解説と照らし合わせましょう。
問 8
8 点Recombinant DNA technology involves various enzymes and techniques to manipulate genes.
a) Describe how reverse transcriptase is used to produce a gene from mRNA.
b) Explain the importance of sticky ends produced by restriction endonucleases when inserting a DNA fragment into a vector.
c) In the Polymerase Chain Reaction (PCR), explain why the mixture is heated to \(95^{\circ}C\) and then cooled to \(55^{\circ}C\) before being heated to \(72^{\circ}C\).
d) Suggest one ethical concern regarding the use of gene therapy in humans.
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